Genetic variation of phosphoprotein gene of avirulent Newcastle disease virus of duck origin in China 我国几种野鸭和家鸭遗传多样性的SSR分析鸭源新城疫病毒P基因遗传变异分析
Expression and antigenicity analysis of P gene in Sendai virus 仙台病毒P基因的原核表达及抗原性分析
Analysis on Mutation of S gene and P gene of Hepatitis B Virus in Two Counties of Sichuan Province 四川省两县(区)乙型肝炎病毒S基因和P基因变异分析
Fractional Expression of P Protein of Hepatitis B Virus in E. coli and Purification of Expressed Product 乙型肝炎病毒P蛋白在大肠杆菌中的分段表达及纯化
The Function of P Gene of Newcastle Disease Virus and Its Role in Pathogenicity 反向遗传技术研究新城疫病毒P基因功能及其对病毒毒力的影响
Isotopic diagnosis and molecular identification of cucumber mosaic virus and satellite RNA infecting tomato in Shanghai 侵染上海番茄的黄瓜花叶病毒及其卫星RNA的~(32)P分子标记杂交检测及分子鉴定
AIM: To investigate the specific humoral and cellular immune response induced by prime-boost immunization of HBsAg protein vaccine ( P), recombinant vaccinia virus vaccine ( V) and DNA vaccine ( D) in mice. 目的:了解HBsAg的蛋白疫苗(P)、痘苗病毒疫苗(V)、DNA疫苗(D)联合免疫小鼠诱导的特异性体液和细胞免疫应答。
Objective To establish a convenient method for detecting YMDD mutations in polymerase gene ( P gene) of hepatitis B virus ( HBV) and to evaluate the effect of lamivudine on these mutations. 目的建立简便易行的检测乙型肝炎病毒(HBV)多聚酶基因(P基因)上YMDD变异株的方法,评价运用拉米夫定治疗慢性乙型肝炎患者对此变异的影响。
The cDNA fragments were cloned and then sequenced. The nucleotide sequence of P and M gene ORF and their deduced amino acid sequence were compared with that of other rabies virus strains and rabies-related virus strains published previously. 将所测定的P和M基因的ORF核苷酸序列和推定的氨基酸序列与国内外公开发表的其它狂犬病病毒固定毒株、狂犬病相关病毒的相应部分进行同源性比较。
A DNA hybridization assay for human and duck hepatitis B virus in serum and liver specimens is described using a nonradioactive Digoxigenin labelled DNA probe, and compared with 32P probe. 应用异羟基洋地黄毒甙元标记的探针,检测了人和鸭的血清及肝脏中的乙型肝炎病毒核酸,并与~(32)P标记的同位素探针做了比较。
Using the [ a-~ ( 32) p] dCTP labelled pEH 920 DNA as the probe, dengue virus RNA in the supernatants of infected C6/ 36 mosquito cells have been detected by DNA-RNA dot-spot hybridization. 以[a-~(32)P]dCTP按缺口转译法标记pEH920DNA作为探针,以感染病毒的蚊细胞c6/36培养上清作标本,应用DNA-RNA斑点杂交法检测了登革病毒核酸。
Construction and Package of Antisense Thrombin Receptor and p~ ( 21) Co-expression Adeno-associated Virus Vector 携带反义凝血酶受体和p21双基因共表达腺病毒伴随病毒载体的构建与包装
Results indicated that: at6weeks of age, tibial Ca and P, antibody titer against Newcastle disease virus increased with the in-creasing of dietary VA. 结果:6周末胫骨Ca和P、新城疫抗体效价随日粮VA水平的提高显著增加(P<0.01)。
The metabolic products of the rod in the culture fluids can induce early antigen and capsul antigen of Epstein-Barr virus in human lymphoblastic Raji cells and P_3HR-I cells. 此菌在液体培养基中的代谢产物,具有诱导人类淋巴母细胞Raji细胞和P3HR-l细胞中的EB病毒的早期抗原(EA)和壳抗原(VCA)的作用。
The infection rate of HPV-16 was higher than that of HPH-18 in cervical squamous cell carcinoma, there was significant difference between the 2 virus types ( P < 0. 01); 核酸杂交结果表明,宫颈鳞癌中,HPV-16的感染率较高,HPV-18的感染率较低,二者比较差异有显著性(P<0.01);
P 1 virus isolate with rod particle was isolated from peach trees with red leaf disease symptom. 从北京地区具有红叶及叶片侧脉失绿等综合症状的桃病株上分离到一种杆状病毒分离物P-1,初步鉴定为一TMV株系;
The difference of the positive rate of measles virus to antibody among the different age monkeys was not remarkable ( P> 0. 01). 不同性别及不同年龄猴麻疹病毒抗体阳性率没有显著性差异(P>0.01)。
ENA of the virus was extracted by phenol-chloroform and precipitated by ethanol. cDNA probe was labeled by 33P according to nick translation method. Hybridization was carrying on under 42 ℃. 采用酚-氯仿提取法和乙醇沉淀法提取标本中的RNA,cDNA探针以缺口翻译法标记~(32)P同位素,在42℃条件下进行cDNA-RNA分子杂交。
Small interference RNA mixtures ( siRNA Cocktails) of Rabies virus ( RV) N gene, P gene and G gene were prepared by digesting long double-stranded RNA ( dsRNA) with RNase ⅲ. 采用RNaseⅢ消化长片段双链RNA的方法,制备了狂犬病毒N基因、P基因和G基因小干扰RNA库(siRNACocktail)。
Objective To explore the point mutation in hepatitis B virus polymerase ( HBV P) gene in HBV-infected patients resistant to lamivudine. 目的探讨拉米夫定耐药患者乙型肝炎病毒聚合酶(HBVP)基因点突变。
Results It was found that the total virus positive rate was 60.4% with significant difference ( P < 0.01) among these three groups. Respiratory syncytial virus ( RSV) was the most common pathogen in seven respiratory viruses. 结果3组患儿病毒检测总阳性率为60.4%,各组患儿病毒检测阳性率差异有显著性(P<0.01),但均以呼吸道合胞病毒(RSV)为主,其他病毒阳性率很低。